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Genetically modified organism

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Genetically modified organism

GloFish, the first genetically modified animal to be sold as a pet

A genetically modified organism (GMO) is any organism whose

This article focuses on what organisms have been genetically engineered, and for what purposes. The article on controversies.


  • Production 1
  • History 2
  • Uses 3
    • Microbes 3.1
    • Plants 3.2
      • Transgenic plants 3.2.1
        • Genetically modified crops
      • Cisgenic plants 3.2.2
    • Mammals 3.3
      • Research use 3.3.1
      • Producing human therapeutics 3.3.2
      • Production or food quality traits 3.3.3
      • Human gene therapy 3.3.4
    • Insects 3.4
      • Fruit flies 3.4.1
      • Mosquitoes 3.4.2
      • Bollworms 3.4.3
    • Aquatic life 3.5
      • Cnidarians 3.5.1
      • Fish 3.5.2
  • Regulation 4
  • Controversy 5
  • Recognition of the originators of GM crops 6
  • See also 7
  • References 8
  • External links 9


Genetic modification involves the mutation, insertion, or deletion of genes. Inserted genes usually come from a different species in a form of horizontal gene-transfer. In nature this can occur when exogenous DNA penetrates the cell membrane for any reason. To do this artificially may require:

  • attaching the genes to a virus
  • physically inserting the extra DNA into the nucleus of the intended host with a very small syringe
  • with the use of electroporation (that is, introducing DNA from one organism into the cell of another by use of an electric pulse)
  • with very small particles fired from a gene gun.[1][2][3]

Other methods exploit natural forms of gene transfer, such as the ability of Agrobacterium to transfer genetic material to plants,[4] or the ability of lentiviruses to transfer genes to animal cells.[5]


Humans have been Paul Berg in 1972.[9][10]


GMOs are used in biological and medical research, production of species into another. For example, a gene from a jellyfish, encoding a fluorescent protein called GFP, can be physically linked and thus co-expressed with mammalian genes to identify the location of the protein encoded by the GFP-tagged gene in the mammalian cell. Such methods are useful tools for biologists in many areas of research, including those who study the mechanisms of human and other diseases or fundamental biological processes in eukaryotic or prokaryotic cells.


Bacteria were the first organisms to be modified in the laboratory, due to their simple genetics.[11]

They continue to be important model organisms for experiments in genetic engineering. In the field of synthetic biology, they have been used to test various synthetic approaches, from synthesizing genomes to creating novel nucleotides.[12][13][14]

These organisms are now used for several purposes, and are particularly important in producing large amounts of pure human proteins for use in medicine.[15]

Genetically modified bacteria are used to produce the protein insulin to treat diabetes.[16] Similar bacteria have been used to produce biofuels,[17] clotting factors to treat haemophilia,[18] and human growth hormone to treat various forms of dwarfism.[19][20]

In addition, various genetically engineered micro-organisms are routinely used as sources of enzymes for the manufacture of a variety of processed foods. These include alpha-amylase from bacteria, which converts starch to simple sugars, chymosin from bacteria or fungi, which clots milk protein for cheese making, and pectinesterase from fungi, which improves fruit juice clarity.[21]


Transgenic plants

Kenyans examining insect-resistant transgenic Bt corn

Transgenic plants have been engineered for scientific research, to create new colours in plants, and to create different crops.

In research, plants are engineered to help discover the functions of certain genes. One way to do this is to knock out the gene of interest and see what phenotype develops. Another strategy is to attach the gene to a strong promoter and see what happens when it is over expressed. A common technique used to find out where the gene is expressed is to attach it to GUS or a similar reporter gene that allows visualisation of the location.[22]'

Suntory "blue" rose

After thirteen years of collaborative research, an Australian company – [24]

Simple plants and plant cells have been genetically engineered for production of biopharmaceuticals in bioreactors as opposed to cultivating plants in open fields. Work has been done with duckweed Lemna minor,[27] the algae Chlamydomonas reinhardtii[28] and the moss Physcomitrella patens.[29][30] An Israeli company, Protalix, has developed a method to produce therapeutics in cultured transgenic carrot and tobacco cells.[31] Protalix and its partner, Pfizer, received FDA approval to market its drug Elelyso, a treatment for Gaucher's disease, in 2012.[32]

Genetically modified crops

In agriculture, currently marketed genetically engineered crops have traits such as resistance to pests, resistance to herbicides, increased nutritional value, or production of valuable goods such as drugs (pharming). Products under development include crops that are able to thrive in environmental conditions outside the species' native range or in changed conditions in their range (e.g. drought or salt resistance). Products that existed and have been withdrawn include those with extended product shelf life, such as the Flavr-savr tomato.

Since the first commercial cultivation of genetically modified plants in 1996, they have been modified to be tolerant to the herbicides glufosinate and glyphosate, to be resistant to virus damage (as in Ringspot virus-resistant GM papaya grown in Hawaii), and to produce the Bt toxin, an insecticide that is documented as non-toxic to mammals.[33][34] Plants, including algae, jatropha, maize, and poplars,[35] have been genetically modified for use in producing fuel, known as biofuel.

Second- and third-generation GM crops are on the market and under development with improved nutrition profiles and increased yields or ability to thrive in difficult environments.[36] GM oilseed crops on the market today offer improved oil profiles for processing or healthier edible oils.[37] Other examples include:

  • A genetically modified cassava with lower cyanogen glucosides and enhanced with protein and other nutrients;[38]
  • Golden rice, which was developed by the International Rice Research Institute (IRRI) and has been discussed as a possible cure for Vitamin A deficiency;[39]
  • A vitamin-enriched corn derived from South African white corn variety;[40]
  • Camelina sativa that accumulates high levels of oils similar to fish oils.[41][42]

For discussions of issues about GM crops and GM food, see the Controversies section below and the article on genetically modified food controversies.

Cisgenic plants

Cisgenesis, sometimes also called intragenesis, is a product designation for a category of genetically engineered plants. A variety of classification schemes have been proposed[43] that order genetically modified organisms based on the nature of introduced genotypical changes rather than the process of genetic engineering.

While some genetically modified plants are developed by the introduction of a gene originating from distant, sexually incompatible species into the host genome, cisgenic plants contain genes that have been isolated either directly from the host species or from sexually compatible species. The new genes are introduced using recombinant DNA methods and gene transfer. Some scientists hope that the approval process of cisgenic plants might be simpler than that of proper transgenics,[44] but it remains to be seen.[45]


Some chimeras, like the blotched mouse shown, are created through genetic modification techniques like gene targeting.

  • Everything you wanted to know about GM organisms — Provided by New Scientist.
  • Transgenic Organism Research
  • International Society for Transgenic Technologies (ISTT)
  • GMO-Compass: Information on genetically modified organisms
  • Co-Extra: Research on co-existence and traceability of GM and non-GM supply chains
  • ISAAA Knowledge Center: Information on genetically modified organisms
  • EFSA GMO Panel deliberations on the Austrian report "Biological effects of transgenic maize NK603 x MON 810 fed in long term reproduction studies in mice" (Part of the minutes of the plenary meeting held on 3–4 December 2008, see on page 9)
  • Do Seed Companies Control GM Crop Research?, Scientific American, August 2009.

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  129. ^ Andrew Pollack (19 June 2013). "Executive at Monsanto Wins Global Food Honor". The New York Times. Retrieved 20 June 2013. 


See also

On 19 June 2013 the leaders of the three research teams that first applied genetic engineering to crops, Robert Fraley of Monsanto; Marc Van Montagu of Ghent University in Belgium and founder of Plant Genetic Systems and Crop Design; and Mary-Dell Chilton of the University of Washington and Washington University in St. Louis and Syngenta, were awarded with the World Food Prize. The prize, of $250,000, is awarded to people who improve the "quality, quantity or availability" of food in the world. The three competing teams first presented their results in January 1983.[129]

Recognition of the originators of GM crops

Opponents of genetically modified food such as the advocacy groups Union of Concerned Scientists, and Greenpeace claim risks have not been adequately identified and managed, and they have questioned the objectivity of regulatory authorities. Some health groups say there are unanswered questions regarding the potential long-term impact on human health from food derived from GMOs, and propose mandatory labeling[122][123] or a moratorium on such products.[124][125][126] Concerns include contamination of the non-genetically modified food supply,[127] effects of GMOs on the environment and nature,[124][126] the rigor of the regulatory process,[125][128] and consolidation of control of the food supply in companies that make and sell GMOs.[124]

There is broad scientific consensus that food on the market derived from GM crops poses no greater risk than conventional food.[115][116][117][118] No reports of ill effects have been proven in the human population from ingesting GM food.[115][119][120][121] Although labeling of GMO products in the marketplace is required in many countries, it is not required in the United States and no distinction between marketed GMO and non-GMO foods is recognized by the US FDA. In an May 2014 article in The Economist it was argued that, while GM foods could potentially help feed 842 million malnourished people globally, laws such as those being considered by Vermont's governor, Peter Shumlin, to require labeling of foods containing genetically modified ingredients, could have the unintended consequence of interrupting the benign process of spreading GM technologies to impoverished countries that suffer with food security problems.[115]

There is controversy over GMOs, especially with regard to their use in producing food. The dispute involves consumers, biotechnology companies, governmental regulators, non-governmental organizations, and scientists. The key areas of controversy related to GMO food are whether GM food should be labeled, the role of government regulators, the effect of GM crops on health and the environment, the effect on pesticide resistance, the impact of GM crops for farmers, and the role of GM crops in feeding the world population.


The regulation of genetic engineering concerns the approaches taken by governments to assess and manage the risks associated with the use of genetically modified crops and genetically modified fish. There are differences in the regulation of GMOs between countries, with some of the most marked differences occurring between the USA and Europe.[111] Regulation varies in a given country depending on the intended use of the products of the genetic engineering. For example, a crop not intended for food use is generally not reviewed by authorities responsible for food safety.[112] The European Union differentiates between approval for cultivation within the EU and approval for import and processing. While only a few GMOs have been approved for cultivation in the EU a number of GMOs have been approved for import and processing.[113] The cultivation of GMOs has triggered a debate about coexistence of GM and nonGM crops. Depending on the coexistence regulations, incentives for cultivation of GM crops differ.[114]


Several academic groups have been developing GM zebrafish to detect aquatic pollution. The lab that originated the GloFish discussed above originally developed them to change color in the presence of pollutants, to be used as environmental sensors.[106][107] A lab at University of Cincinnati has been developing GM zebrafish for the same purpose,[108][109] as has a lab at Tulane University.[110]

Genetically modified fish have been developed with promoters driving an over-production of "all fish" growth hormone for use in the aquaculture industry to increase the speed of development and potentially reduce fishing pressure on wild stocks. This has resulted in dramatic growth enhancement in several species, including salmon,[102] trout[103] and tilapia.[104] AquaBounty, a biotechnology company working on bringing a GM salmon to market, claims that their GM AquAdvantage salmon can mature in half the time it takes non-GM salmon and achieves twice the size.[70] AquaBounty has applied for regulatory approval to market their GM salmon in the US. As of December 2012 the application was still pending.[71][105]

The GloFish is a patented[99] brand of genetically modified (GM) fluorescent zebrafish with bright red, green, and orange fluorescent color. Although not originally developed for the ornamental fish trade, it became the first genetically modified animal to become publicly available as a pet when it was introduced for sale in 2003.[100] They were quickly banned for sale in California.[101]

Genetically engineered fish are widely used in basic research in genetics and development. Two species of fish, zebrafish and medaka, are most commonly modified because they have optically clear chorions (shells), rapidly develop, and the 1-cell embryo is easy to see and microinject with transgenic DNA.[98]

GM fish are used for scientific research and as pets, and are being considered for use as food and as aquatic pollution sensors.


evolution of immunity and certain developmental processes. An important technical breakthrough was the development of procedures for generation of stably transgenic hydras and sea anemones by embryo microinjection.[97]


Aquatic life

A strain of Pectinophora gossypiella (Pink bollworm) has been genetically engineered to express a red fluorescent protein. This allows researchers to monitor bollworms that have been sterilized by radiation and released in order to reduce bollworm infestation. The strain has been field tested for over three years and has been approved for release.[94][95][96]


In 2010, scientists created "malaria-resistant mosquitoes" in the laboratory.[87][88][89] The World Health Organization estimated that Malaria killed almost one million people in 2008.[90] Genetically modified male mosquitoes containing a lethal gene have been developed in order to combat the spread of Dengue fever.[91] Aedes aegypti mosquitoes, the single most important carrier of dengue fever, were reduced by 80% in a 2010 trial of these GM mosquitoes in the Cayman Islands.[92][93] Between 50 and 100 million people are affected by Dengue fever every year and 40,000 people die from it.[94]


. vertebrates Fruit flies are often preferred over other animals due to their short life cycle, low maintenance requirements, and relatively simple genome compared to many [86] In biological research, transgenic fruit flies (

Fruit flies


Gene therapy,[73] uses genetically modified viruses to deliver genes that can cure disease in humans. Although gene therapy is still relatively new, it has had some successes. It has been used to treat genetic disorders such as severe combined immunodeficiency,[74] and Leber's congenital amaurosis.[75] Treatments are also being developed for a range of other currently incurable diseases, such as cystic fibrosis,[76] sickle cell anemia,[77] Parkinson's disease,[78][79] cancer,[80][81][82] diabetes,[83] heart disease[84] and muscular dystrophy.[85] Current gene therapy technology only targets the non-reproductive cells meaning that any changes introduced by the treatment can not be transmitted to the next generation. Gene therapy targeting the reproductive cells—so-called "Germ line Gene Therapy"—is very controversial and is unlikely to be developed in the near future.

Human gene therapy

Genetically modified fish have been developed with promoters driving an over-production of growth hormone for use in the aquaculture industry to increase the speed of development and potentially reduce fishing pressure on wild stocks. AquaBounty, a biotechnology company working on bringing a GM salmon to market, claims that their GM AquAdvantage salmon can mature in half the time it takes non-GM salmon and achieves twice the size.[70] AquaBounty has applied for regulatory approval to market their GM salmon in the US. As of May 2012 the application was still pending.[71] On 25 November 2013 Canada approved commercial scale production and export of GM Salmon eggs but they are not approved for human consumption in Canada.[72]

Goats have been genetically engineered to produce milk with strong spiderweb-like silk proteins in their milk.[69]

In 2006, a pig was engineered to produce omega-3 fatty acids through the expression of a roundworm gene.[68]

In 2011, Chinese scientists generated dairy cows genetically engineered with genes from human beings in order to produce milk that would be the same as human breast milk.[64] This could potentially benefit mothers who cannot produce breast milk but want their children to have breast milk rather than formula. Aside from milk production, the researchers claim these transgenic cows to be identical to regular cows.[65] Two months later scientists from Argentina presented Rosita, a transgenic cow incorporating two human genes, to produce milk with similar properties as human breast milk.[66] In 2012, researchers from New Zealand also developed a genetically engineered cow that produced allergy-free milk.[67]

Enviropig was a genetically enhanced line of Yorkshire pigs in Canada created with the capability of digesting plant phosphorus more efficiently than conventional Yorkshire pigs. The project ended in 2012.[61][62] These pigs produced the enzyme phytase, which breaks down the indigestible phosphorus, in their saliva. The enzyme was introduced into the pig chromosome by pronuclear microinjection. With this enzyme, the animal is able to digest cereal grain phosphorus.[61][63] The use of these pigs would reduce the potential of water pollution since they excrete from 30 to 70.7% less phosphorus in manure depending upon the age and diet.[61][63] The lower concentrations of phosphorus in surface runoff reduces algal growth, because phosphorus is the limiting nutrient for algae.[61] Because algae consume large amounts of oxygen, it can result in dead zones for fish.

Production or food quality traits

Within the field known as pharming, intensive research has been conducted to develop transgenic animals that produce biotherapeutics.[59] On 6 February 2009, the U.S. Food and Drug Administration approved the first human biological drug produced from such an animal, a goat. The drug, ATryn, is an anticoagulant which reduces the probability of blood clots during surgery or childbirth. It is extracted from the goat's milk.[60]

Producing human therapeutics

In 2009, scientists in Japan announced that they had successfully transferred a gene into a primate species (marmosets) and produced a stable line of breeding transgenic primates for the first time.[56][57] Their first research target for these marmosets was Parkinson's disease, but they were also considering Amyotrophic lateral sclerosis and Huntington's disease.[58]

Scientists have genetically engineered several organisms, including some mammals, to include photoreceptor cells, and other topics.[53] In 2011 a Japanese-American Team created green-fluorescent cats in order to find therapies for HIV/AIDS and other diseases[54] as Feline immunodeficiency virus (FIV) is related to HIV.[55]

Genetically modified (genetically engineered) animals are becoming more vital to the discovery and development of cures and treatments for many serious diseases. By altering the DNA or transferring DNA to an animal, we can develop certain proteins that may be used in medical treatment. Stable expressions of human proteins have been developed in many animals, including sheep, pigs, and rats. Human-alpha-1-antitrypsin,[51] which has been tested in sheep and is used in treating humans with this deficiency and transgenic pigs with human-histo-compatibility have been studied in the hopes that the organs will be suitable for transplant with less chances of rejection.

Transgenic animals are used as experimental models to perform phenotypic and for testing in biomedical research.[50]

Research use

  1. to research human diseases (for example, to develop animal models for these diseases);
  2. to produce industrial or consumer products (fibres for multiple uses);
  3. to produce products intended for human therapeutic use (pharmaceutical products or tissue for implantation);
  4. to enrich or enhance the animals' interactions with humans (hypo-allergenic pets);
  5. to enhance production or food quality traits (faster growing fish, pigs that digest food more efficiently);
  6. to improve animal health (disease resistance)[49]

Genetically modified animals currently being developed can be placed into six different broad classes based on the intended purpose of the genetic modification:

The first transgenic (genetically modified) animal was produced by injecting DNA into mouse embryos then implanting the embryos in female mice.[48]

[47] and Richard Palmiter developed the techniques responsible for transgenic mice, rats, rabbits, sheep, and pigs in the early 1980s, and established many of the first transgenic models of human disease, including the first carcinoma caused by a transgene. The process of genetically engineering animals is a slow, tedious, and expensive process. However, new technologies are making genetic modifications easier and more precise.Ralph L. Brinster [46]

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