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Plos Biology : Intravital Observation of Plasmodium Berghei Sporozoite Infection of the Liver, Volume 3

By Egwang, Thomas

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Book Id: WPLBN0003924075
Format Type: PDF eBook :
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Reproduction Date: 2015

Title: Plos Biology : Intravital Observation of Plasmodium Berghei Sporozoite Infection of the Liver, Volume 3  
Author: Egwang, Thomas
Volume: Volume 3
Language: English
Subject: Journals, Science, Biology
Collections: Periodicals: Journal and Magazine Collection, PLoS Biology
Historic
Publication Date:
Publisher: Plos

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Egwang, T. (n.d.). Plos Biology : Intravital Observation of Plasmodium Berghei Sporozoite Infection of the Liver, Volume 3. Retrieved from http://ebook2.worldlibrary.net/


Description
Description : Plasmodium sporozoite invasion of liver cells has been an extremely elusive event to study. In the prevailing model, sporozoites enter the liver by passing through Kupffer cells, but this model was based solely on incidental observations in fixed specimens and on biochemical and physiological data. To obtain direct information on the dynamics of sporozoite infection of the liver, we infected live mice with red or green fluorescent Plasmodium berghei sporozoites and monitored their behavior using intravital microscopy. Digital recordings show that sporozoites entering a liver lobule abruptly adhere to the sinusoidal cell layer, suggesting a high-affinity interaction. They glide along the sinusoid, with or against the bloodstream, to a Kupffer cell, and, by slowly pushing through a constriction, traverse across the space of Disse. Once inside the liver parenchyma, sporozoites move rapidly for many minutes, traversing several hepatocytes, until ultimately settling within a final one. Migration damage to hepatocytes was confirmed in liver sections, revealing clusters of necrotic hepatocytes adjacent to structurally intact, sporozoite-infected hepatocytes, and by elevated serum alanine aminotransferase activity. In summary, malaria sporozoites bind tightly to the sinusoidal cell layer, cross Kupffer cells, and leave behind a trail of dead hepatocytes when migrating to their final destination in the liver.

 

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