World Library  


Add to Book Shelf
Flag as Inappropriate
Email this Book

Plos One : Identification of a Plant Viral Rna Genome in the Nucleus, Volume 7

By Mukhopadhyay, Partha

Click here to view

Book Id: WPLBN0003938914
Format Type: PDF eBook :
File Size:
Reproduction Date: 2015

Title: Plos One : Identification of a Plant Viral Rna Genome in the Nucleus, Volume 7  
Author: Mukhopadhyay, Partha
Volume: Volume 7
Language: English
Subject: Journals, Science, Medical Science
Collections: Periodicals: Journal and Magazine Collection (Contemporary)
Historic
Publication Date:
Publisher: Plos

Citation

APA MLA Chicago

Mukhopadhyay, P. (n.d.). Plos One : Identification of a Plant Viral Rna Genome in the Nucleus, Volume 7. Retrieved from http://ebook2.worldlibrary.net/


Description
Description : Viruses contain either DNA or RNA as genomes. DNA viruses replicate within nucleus, while most RNA viruses, especially (+)- sense single-stranded RNA, replicate and are present within cytoplasm. We proposed a new thought that is contrary to the common notion that (+)-sense single-stranded RNA viruses are present only in the cytoplasm. In this study, we question whether the genome of a plant RNA virus (non-retroviral) is present in the nucleus of infected cells? Hibiscus chlorotic ringspot virus (HCRSV) RNA was detected in the nucleus of infected cells, as shown by fluorescent in situ hybridization. Western blot using anti-histone 3 and anti-phosphoenolpyruvate carboxylase showed that nuclei were highly purified from mock and HCRSV-infected kenaf (Hibiscus cannabilis L.) leaves, respectively. The p23 and HCRSV coat protein (CP) coding regions were both amplified from total RNA extracted from isolated nuclei. Viral RNA in the nucleus may be used to generate viral microRNAs (vir-miRNAs), as five putative vir-miRNAs were predicted from HCRSV using the vir-miRNAs prediction database. The vir-miRNA (hcrsv-miR-H1-5p) was detected using TaqManH stem-loop real-time PCR, and by northern blot using DIG-end labeled probe in HCRSV-infected kenaf leaves. Finally, a novel nuclear localization signal (NLS) was discovered in p23 of HCRSV. The NLS interacts with importin a and facilitates viral RNA genome to enter nucleus. We demonstrate the presence of a (+)-sense single-stranded viral RNA within nucleus.

 

Click To View

Additional Books


  • Plos One : Morphological and Metabolic C... (by )
  • Plos One : Effect of Sodium Bicarbonate ... (by )
  • Plos One : Differential Effects of Topic... (by )
  • Plos One : the Transcriptional Heat Shoc... (by )
  • Plos One : Foods and Dietary Patterns Th... (by )
  • Plos One : Impacts of Brain Serotonin De... (by )
  • Plos One : Comparative Lipid Profiling o... (by )
  • Plos One : Microfabricated Modular Scale... (by )
  • Plos One : Ermani is a Target of Mir-125... (by )
  • Plos One : Anti-inflammatory and Vasopro... (by )
  • Plos One : Tobacco Mosaic Virus in the L... (by )
  • Plos One : the Difference in Prognostic ... (by )
Scroll Left
Scroll Right

 



Copyright © World Library Foundation. All rights reserved. eBooks from World eBook Library are sponsored by the World Library Foundation,
a 501c(4) Member's Support Non-Profit Organization, and is NOT affiliated with any governmental agency or department.